Human Protein SSX1 (SSX1) ELISA Kit

SSX1 belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. They are also capable of eliciting spontaneously humoral and cellular immune responses in cancer patients, and are potentially useful targets in cancer vaccine-based immunotherapy.This translocation results in the fusion of the synovial sarcoma translocation gene on chromosome 18 to one of the SSX genes on chromosome X. The encoded hybrid proteins are probably responsible for transforming activity.Expressed at high level in the testis. Expressed at low level in thyroid. Not detected in tonsil, colon, lung, spleen, prostate, kidney, striated and smooth muscles. Detected in rhabdomyosarcoma and fibrosarcoma cell lines. Not detected in mesenchymal and epithelial cell lines.

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Human Protein SSX1 (SSX1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SSX1. No significant cross-reactivity or interference between Human SSX1 and analogues was observed.

This Human Protein SSX1 (SSX1) ELISA Kit employs a two-site sandwich ELISA to quantitate SSX1 in samples. An antibody specific for SSX1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySSX1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SSX1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SSX1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.