Human Protein phosphatase Slingshot homolog 1 (SSH1) ELISA Kit

The ADF (actin-depolymerizing factor) /cofilin family is composed of stimulus-responsive mediators of actin dynamics. ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1) . The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo. Transcript analysis suggested that at least 6 polypeptides would be produced from these genes. SSH1 encodes 3 isoforms, SSH1L (1,049 amino acids), SSH1S (692 amino acids), and SSH1B (148 amino acids), and SSH2 encodes 2 isoforms, SSH2 (449 amino acids) and SSH2B (195 amino acids) . The SSH3 protein has 471 amino acids. Besides the catalytic domain, 2 other domains are conserved between Drosophila ssh and the human SSHs and are unique to the SSH family.

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Human Protein phosphatase Slingshot homolog 1 (SSH1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SSH1. No significant cross-reactivity or interference between Human SSH1 and analogues was observed.

This Human Protein phosphatase Slingshot homolog 1 (SSH1) ELISA Kit employs a two-site sandwich ELISA to quantitate SSH1 in samples. An antibody specific for SSH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySSH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SSH1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SSH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.