Human Speckle-type POZ protein-like (SPOPL) ELISA Kit

SPOPL Belongs to the Tdpoz family.Contains 1 BTB (POZ) domain.Contains 1 MATH domain.TDPOZ, a family of bipartite animal and plant proteins that contain the TRAF (TD) and POZ/BTB domains. The Tdpoz gene cluster is mapped on chromosome 3 at 3F2.1-2.2. RT-PCR experiments and a search of expressed sequence tag (EST) databases show that the Tdpoz1-5 genes are transcribed in early embryos, particularly at the two-cell stage. TDPOZ proteins form a new protein family on the basis of similar protein domain organization. TDPOZ proteins may be nuclear scaffold proteins probably involved in transcription regulation in early development and other cellular processes.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Speckle-type POZ protein-like (SPOPL) ELISA Kit has high sensitivity and excellent specificity for detection of Human SPOPL. No significant cross-reactivity or interference between Human SPOPL and analogues was observed.

This Human Speckle-type POZ protein-like (SPOPL) ELISA Kit employs a two-site sandwich ELISA to quantitate SPOPL in samples. An antibody specific for SPOPL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPOPL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPOPL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPOPL bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.