Human Speckle-type POZ protein (SPOP) ELISA Kit

SPOP encodes a protein that may modulate the transcriptional repression activities of death-associated protein 6 (DAXX), which interacts with histone deacetylase, core histones, and other histone-associated proteins. In mouse, the encoded protein binds to the putative leucine zipper domain of macroH2A1.2, a variant H2A histone that is enriched on inactivated X chromosomes. The BTB/POZ domain of this protein has been shown in other proteins to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes. Alternative splicing of this gene results in multiple transcript variants encoding the same protein.The SPOP gene encodes a 374-amino acid polypeptide that contains a poxvirus and zinc finger (POZ) domain and an evolutionarily conserved N-terminal region.

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Human Speckle-type POZ protein (SPOP) ELISA Kit has high sensitivity and excellent specificity for detection of Human SPOP. No significant cross-reactivity or interference between Human SPOP and analogues was observed.

This Human Speckle-type POZ protein (SPOP) ELISA Kit employs a two-site sandwich ELISA to quantitate SPOP in samples. An antibody specific for SPOP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPOP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPOP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPOP bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.