Human Spondin-2 (SPON2) ELISA Kit

The full-length SPON2 cDNA encodes a 331-amino acid protein with a domain organization similar to those of zebrafish mindin-1/mindin-2 and F-spondin (SPON1) : a hydrophobic signal sequence in the N terminus, an FS1 domain, an FS2 domain, and a thrombospondin type I repeat. The mouse mindin protein, which is 85% identical to the human protein, is a conserved member of a family of secreted extracellular matrix proteins. RNA blot analysis detected expression in a variety of tissues, with abundant expression in lung and lymphoid tissues. Immunoblot blot analysis showed expression of a 42-kD protein under reducing conditions and expression of dimers and oligomers in a concentration-dependent manner under nonreducing conditions.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Spondin-2 (SPON2) ELISA Kit has high sensitivity and excellent specificity for detection of Human SPON2. No significant cross-reactivity or interference between Human SPON2 and analogues was observed.

This Human Spondin-2 (SPON2) ELISA Kit employs a two-site sandwich ELISA to quantitate SPON2 in samples. An antibody specific for SPON2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPON2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPON2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPON2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.