Human SPOC domain-containing protein 1 (SPOCD1) ELISA Kit

SPOCD1 Contains 1 SPOC domain. Contains 1 TFIIS central domain.The Split ends (Spen) family are large proteins characterised by N-terminal RNA recognition motifs (RRMs) and a conserved SPOC (Spen paralog and ortholog C-terminal) domain.The widely distributed DIO (Death inducer-obliterator) family is related to cancer and apoptosis and offers new clues about SPOC domain functionality. TFIIS is a transcription elongation factor, which directly binds the transcription motor, RNA Polymerase II and allows it to read through various transcription arrest sites. TFIIS, a transcription elongation factor, and its affect on proliferation of cancer cells in culture. Knockdown of TFIIS reduced cancer cell proliferation in breast, lung and pancreatic cancer cell lines.

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Human SPOC domain-containing protein 1 (SPOCD1) ELISA Kit has high sensitivity and excellent specificity for detection of Human SPOCD1. No significant cross-reactivity or interference between Human SPOCD1 and analogues was observed.

This Human SPOC domain-containing protein 1 (SPOCD1) ELISA Kit employs a two-site sandwich ELISA to quantitate SPOCD1 in samples. An antibody specific for SPOCD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPOCD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPOCD1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPOCD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.