Human Protein spinster homolog 2 (SPNS2) ELISA Kit

SPNS2 contains 548 amino acids and shares significant homology with zebrafish Spns2. Kawahara et al. (2009) showed that zebrafish Spns2, which contains 12 transmembrane domains, was expressed at the membrane of transfected Chinese hamster ovary cells. Overexpression of either zebrafish or human SPNS2 increased the release of sphingosine-1-phosphate (S1P) into the culture medium. Kawahara et al. (2009) concluded that SPNS2 functions as an S1P transporter in S1P secretion.an N-ethyl-N-nitrosurea-induced arg153-to-ser mutation in zebrafish Spns2 led to cardiac bifida. Expression of human SPNS2, but not human SPNS1 , reversed the defect. The defect was also rescued by S1P injection or by reintroduction of zebrafish Spns2 in extraembryonic tissue yolk syncytial layer.

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Human Protein spinster homolog 2 (SPNS2) ELISA Kit has high sensitivity and excellent specificity for detection of Human SPNS2. No significant cross-reactivity or interference between Human SPNS2 and analogues was observed.

This Human Protein spinster homolog 2 (SPNS2) ELISA Kit employs a two-site sandwich ELISA to quantitate SPNS2 in samples. An antibody specific for SPNS2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPNS2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPNS2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPNS2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.