Human TCDD-inducible poly [ADP-ribose] polymerase (TIPARP) ELISA Kit

TIPARP, encodes a deduced 657-amino acid protein that shares 91.8% and 52.7% sequence identity with mouse and fugu Tiparp proteins, respectively. The proteins in all 3 species contain 3 highly conserved domains: a WWE (trp, trp, glu) domain, a PARP (poly (ADP-ribose) polymerase) -like catalytic domain, and a novel TPH (TIPARP homologous) domain. The N-terminal part of the TPH domain contains a CCCH-type zinc finger. Katoh and Katoh (2003) noted that the deduced proteins of 2 human genes, which they called FLJ226093 (PARP12) and ZAP (ZC3HAV1), contain the same 3 conserved domains and share 27.5% and 26% overall sequence identity with TIPARP, respectively.

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Human TCDD-inducible poly [ADP-ribose] polymerase (TIPARP) ELISA Kit has high sensitivity and excellent specificity for detection of Human TIPARP. No significant cross-reactivity or interference between Human TIPARP and analogues was observed.

This Human TCDD-inducible poly [ADP-ribose] polymerase (TIPARP) ELISA Kit employs a two-site sandwich ELISA to quantitate TIPARP in samples. An antibody specific for TIPARP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTIPARP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TIPARP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TIPARP bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.