Human Transmembrane 4 L6 family member 5 (TM4SF5) ELISA Kit

Like other TM4SF proteins, TM4SF5 is cysteine-rich. TM4SF5 shares 50% amino acid identity with the tumor-associated antigen L6 (TM4SF1) . Northern blot analysis of various normal human tissues, namely intestine, pancreas, stomach, colon, esophagus, bladder, uterus, placenta, and adipose tissue, detected TM4SF5 expression only in intestine and 1 of 14 pancreas samples. TM4SF5 was overexpressed in 13 of 14 pancreatic adenocarcinomas, as compared with both normal pancreatic and chronic pancreatitis tissues. TM4SF5 expression was also found in 2 of 2 gastric cancers, 1 of 2 colon carcinomas, 1 of 1 carcinoma of the papilla vateri, and 1 of 1 soft-tissue sarcoma. TM4SF5 was expressed as an approximately 1-kb transcript in the pancreatic tumors.

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Human Transmembrane 4 L6 family member 5 (TM4SF5) ELISA Kit has high sensitivity and excellent specificity for detection of Human TM4SF5. No significant cross-reactivity or interference between Human TM4SF5 and analogues was observed.

This Human Transmembrane 4 L6 family member 5 (TM4SF5) ELISA Kit employs a two-site sandwich ELISA to quantitate TM4SF5 in samples. An antibody specific for TM4SF5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTM4SF5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TM4SF5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TM4SF5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.