Human Transmembrane protein 165 (TMEM165) ELISA Kit

TMEM165, Belongs to the UPF0016 (TC 9.B.26) family.The Clock gene encodes a basic helix-loop-helix (bHLH) -PAS transcription factor that regulates circadian rhythms in mice. This region contains the complete loci for the Clock and Tpardl (pFT27) genes, as well as the 3' partial locus of the Neuromedin U gene; sequence analysis also suggests the presence of two previously unidentified genes. Finally, a new BAC transgenic line indicates that the genomic region that is sufficient for rescue of the Clock mutant phenotype is no greater than 120 kb and tightly flanks the 3' end of the Clock gene.

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Human Transmembrane protein 165 (TMEM165) ELISA Kit has high sensitivity and excellent specificity for detection of Human TMEM165. No significant cross-reactivity or interference between Human TMEM165 and analogues was observed.

This Human Transmembrane protein 165 (TMEM165) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM165 in samples. An antibody specific for TMEM165 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM165 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM165 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM165 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.