Human Transmembrane protein 55B (TMEM55B) ELISA Kit

TMEM55B catalyzes the degradation of phosphatidylinositol 4,5-bisphosphate (PtdIns-4,5-P2) by removing the 4-phosphate. The deduced 257-amino acid protein contains a central Cx (5) R phosphatase catalytic motif and 2 transmembrane domains near its C terminus, a characteristic of lysosomal transmembrane proteins. RNA dot blot analysis detected expression in all tissues examined. Fluorescence-tagged TMEM55B localized with membrane markers of late endosomes or lysosomes. Endogenous HeLa cell TMEM55B showed a similar distribution.It showed no activity toward other phosphatidylinositol substrates or inositol phosphates tested. Overexpression of TMEM55B in human embryonic kidney cells reduced the total cellular level of PtdIns-4,5-P2.

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Human Transmembrane protein 55B (TMEM55B) ELISA Kit has high sensitivity and excellent specificity for detection of Human TMEM55B. No significant cross-reactivity or interference between Human TMEM55B and analogues was observed.

This Human Transmembrane protein 55B (TMEM55B) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM55B in samples. An antibody specific for TMEM55B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM55B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM55B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM55B bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.