Human Macoilin (TMEM57) ELISA Kit

TMEM57 has 5 domains, including an N-terminal LAG1 domain and a central ERM-myosin filament domain. The LAG1 domain contains 3 putative transmembrane domains. In situ hybridization of mouse embryos showed that Tmem57 was brain specific. It was primarily associated with postmigratory neurons and less so with neural proliferating cells, and Tmem57 expression was progressively downregulated during brain maturation. Fluorescence-tagged Tmem57 outlined the nuclear membrane when expressed in nonneuronal cells. In primary hippocampal neurons, Tmem57 was found in neurites and their terminals, and it partially colocalized with synapsin-1 (SYN1), a presynaptic marker.

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Human Macoilin (TMEM57) ELISA Kit has high sensitivity and excellent specificity for detection of Human TMEM57. No significant cross-reactivity or interference between Human TMEM57 and analogues was observed.

This Human Macoilin (TMEM57) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM57 in samples. An antibody specific for TMEM57 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM57 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM57 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM57 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.