Human Transmembrane protein 59-like (TMEM59L) ELISA Kit

BSMAP, a novel protein expressed specifically in the brain whose gene is localized on chromosome 19p12. BSMAP encoding the full-length open reading frame. Northern blot analysis revealed that BSMAP mRNA is preferentially expressed at a high level in the brain. BSMAP has a putative transmembrane domain and is predicted to be a type-I membrane glycoprotein. Genomic sequence analysis revealed that the gene encoding BSMAP consists of eight exons spanning approximately 8 kb and lies 6 kb away from the gene encoding CLF-1 in a reverse orientation. Although no candidate genetic disorders were found to map either to this precise region of chromosome 19 or to the syntenic region of the mouse genome, the highly specific expression of BSMAP mRNA suggests a role for the protein in CNS function.

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Human Transmembrane protein 59-like (TMEM59L) ELISA Kit has high sensitivity and excellent specificity for detection of Human TMEM59L. No significant cross-reactivity or interference between Human TMEM59L and analogues was observed.

This Human Transmembrane protein 59-like (TMEM59L) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM59L in samples. An antibody specific for TMEM59L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM59L present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM59L is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM59L bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.