Human Transmembrane protein 63A (TMEM63A) ELISA Kit

TMEM63A is a potential multi-pass membrane protein. It belongs to the SPO75/TMEM63 family. The exact function of TMEM63A remains unknown. spo75 mutants displayed a very heterogeneous phenotype. The phenotypes of spores in spo75 cells ranged from those that had little or no spore wall material to ones that had a wild-type appearance. A transmembrane protein is a polytopic protein that spans the entire biological membrane. Transmembrane proteins aggregate and precipitate in water. They require detergents or nonpolar solvents for extraction, although some of them (beta-barrels) can be also extracted using denaturing agents.

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Human Transmembrane protein 63A (TMEM63A) ELISA Kit has high sensitivity and excellent specificity for detection of Human TMEM63A. No significant cross-reactivity or interference between Human TMEM63A and analogues was observed.

This Human Transmembrane protein 63A (TMEM63A) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM63A in samples. An antibody specific for TMEM63A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM63A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM63A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM63A bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.