Human Tropomodulin-3 (TMOD3) ELISA Kit

By RNA dot blot analysis, Conley et al. (2001) confirmed expression of TMOD3, which they designated UTMOD, in all adult and fetal tissues examined. Expression was relatively uniform in all tissues tested, showing less than a 4-fold difference between the highest and lowest levels. Using EST database searches, sequencing, and RT-PCR, Cox and Zoghbi (2000) cloned human and mouse cDNAs corresponding to the TMOD3 gene. Human TMOD3 encodes a deduced 352-amino acid protein. Northern blot analysis on human tissues detected ubiquitous expression of 7 TMOD3 transcripts ranging in size from approximately 1 to 9.5 kb. In the mouse, Tmod3 expression was present as early as embryonic day 7 and continued throughout development.

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Human Tropomodulin-3 (TMOD3) ELISA Kit has high sensitivity and excellent specificity for detection of Human TMOD3. No significant cross-reactivity or interference between Human TMOD3 and analogues was observed.

This Human Tropomodulin-3 (TMOD3) ELISA Kit employs a two-site sandwich ELISA to quantitate TMOD3 in samples. An antibody specific for TMOD3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMOD3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMOD3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMOD3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.