Human Tropomodulin-4 (TMOD4) ELISA Kit

TMOD4 encodes a muscle-specific member of the tropomodulin family of actin-regulatory proteins. The encoded protein caps the pointed end of actin filaments preventing both elongation and depolymerization. The capping activity of this protein is dependent on its association with tropomyosin. Alternatively spliced transcript variants encoding different isoforms have been described. Human TMOD4 encodes a deduced 345-amino acid protein. Northern blot analysis on human tissues detected a prominent 1.3- to 1.5-kb transcript in skeletal muscle. Three faint transcripts were detected: a 6.8-kb transcript in heart and skeletal muscle, and 2.4 and 3.0 transcripts in skeletal muscle.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Tropomodulin-4 (TMOD4) ELISA Kit has high sensitivity and excellent specificity for detection of Human TMOD4. No significant cross-reactivity or interference between Human TMOD4 and analogues was observed.

This Human Tropomodulin-4 (TMOD4) ELISA Kit employs a two-site sandwich ELISA to quantitate TMOD4 in samples. An antibody specific for TMOD4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMOD4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMOD4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMOD4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.