Human Thymosin beta-15A (TMSB15A) ELISA Kit

The use of the serum prostate-specific antigen (PSA) test permits early detection of human prostate cancer; however, early detection has not been accompanied by an improvement in determining which tumors may progress to the metastatic stage. The process of tumor metastasis is a multistage event involving local invasion and destruction of extracellular matrix; intravasation into blood vessels, lymphatics or other channels of transport; survival in the circulation; extravasation out of the vessels into the secondary site; and growth in the new location. Common to many components of the metastatic process is the requirement for tumor cell motility. A well-characterized series of cell lines that showed varying metastatic potential was developed from the Dunning rat prostate carcinoma. The molecule, named thymosin-beta-15 , was found to deregulate motility in prostate cells directly.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Thymosin beta-15A (TMSB15A) ELISA Kit has high sensitivity and excellent specificity for detection of Human TMSB15A. No significant cross-reactivity or interference between Human TMSB15A and analogues was observed.

This Human Thymosin beta-15A (TMSB15A) ELISA Kit employs a two-site sandwich ELISA to quantitate TMSB15A in samples. An antibody specific for TMSB15A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMSB15A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMSB15A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMSB15A bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.