Human Tumor necrosis factor alpha-induced protein 8 (TNFAIP8) ELISA Kit

TNFAIP8 was an early response gene, with expression peaking between 1.5 and 3 hours. TNF-alpha , a proapoptotic protein, also induced TNFAIP8 expression. The deduced 188-amino acid protein has a calculated molecular mass of 21 kD. TNFAIP8 contains a death effector domain (DED) that shares significant similarity with DED II of the FLIP family of cell death regulatory proteins. Northern blot analysis detected a 2.0-kb transcript in most adult and fetal human tissues, with high expression in adult spleen, lymph node, thymus, thyroid, bone marrow, and placenta, and in fetal liver, lung, and kidney. TNFAIP8 was also expressed in all cancer cell lines tested.The deduced protein contains 188 amino acids. In situ hybridization showed weak expression in adult human arteries.

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Human Tumor necrosis factor alpha-induced protein 8 (TNFAIP8) ELISA Kit has high sensitivity and excellent specificity for detection of Human TNFAIP8. No significant cross-reactivity or interference between Human TNFAIP8 and analogues was observed.

This Human Tumor necrosis factor alpha-induced protein 8 (TNFAIP8) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFAIP8 in samples. An antibody specific for TNFAIP8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFAIP8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFAIP8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFAIP8 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.