Human Tumor necrosis factor alpha-induced protein 8-like protein 2 (TNFAIP8L2) ELISA Kit

Sun et al. (2008) cloned mouse Tnfaip8l2, which they called Tipe2, and by database analysis, they identified human TNFAIP8L2. The deduced 184-amino acid human protein shares 94% identity with mouse Tnfaip8l2, shares 53% identity with human TNFAIP8 , and contains a death effector domain (DED) and 6 putative alpha-helices characteristic of DED-containing proteins. Northern blot analysis detected strong expression in mouse thymus, spleen, lymph node, and small intestine, with weak expression in lung, skin, and colon. Tnfaip8l2 was also expressed in murine lymphoid cells and cell lines, in inflamed mouse spinal cord, and in TNF-alpha-stimulated mouse fibroblasts, suggesting that Tnfaip8l2 is preferentially expressed in lymphoid tissue.

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Human Tumor necrosis factor alpha-induced protein 8-like protein 2 (TNFAIP8L2) ELISA Kit has high sensitivity and excellent specificity for detection of Human TNFAIP8L2. No significant cross-reactivity or interference between Human TNFAIP8L2 and analogues was observed.

This Human Tumor necrosis factor alpha-induced protein 8-like protein 2 (TNFAIP8L2) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFAIP8L2 in samples. An antibody specific for TNFAIP8L2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFAIP8L2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFAIP8L2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFAIP8L2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.