Human Tumor necrosis factor receptor superfamily member 21 (TNFRSF21) ELISA Kit

Tumor necrosis factor receptor superfamily, member 21 is a member of the TNF-receptor superfamily. This receptor has been shown to activate NF-κB and MAPK8/JNK, and induce cell apoptosis. Through its death domain, this receptor interacts with TRADD protein, which is known to serve as an adaptor that mediates signal transduction of TNF-receptors. Knockout studies in mice suggested that this gene plays a role in T helper cell activation, and may be involved in inflammation and immune regulation.Highly expressed in heart, brain, placenta, pancreas, lymph node, thymus and prostate. Detected at lower levels in lung, skeletal muscle, kidney, testis, uterus, small intestine, colon, spleen, bone marrow and fetal liver. Very low levels were found in adult liver and peripheral blood leukocytes.

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Human Tumor necrosis factor receptor superfamily member 21 (TNFRSF21) ELISA Kit has high sensitivity and excellent specificity for detection of Human TNFRSF21. No significant cross-reactivity or interference between Human TNFRSF21 and analogues was observed.

This Human Tumor necrosis factor receptor superfamily member 21 (TNFRSF21) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFRSF21 in samples. An antibody specific for TNFRSF21 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFRSF21 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFRSF21 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFRSF21 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.