Human Soluble OX40L (sOX40L) ELISA Kit

SOX40L encodes a cytokine of the tumor necrosis factor (TNF) ligand family. Soluble OX40L functions in T cell antigen-presenting cell (APC) interactions and mediates adhesion of activated T cells to endothelial cells. Polymorphisms in this gene have been associated with Sjogren's syndrome and systemic lupus erythematosus. Alternative splicing results in multiple transcript variants.

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Human Soluble OX40L (sOX40L) ELISA Kit has high sensitivity and excellent specificity for detection of Human TNFSF4. No significant cross-reactivity or interference between Human TNFSF4 and analogues was observed.

This Human Soluble OX40L (sOX40L) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFSF4 in samples. An antibody specific for TNFSF4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFSF4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFSF4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFSF4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.