Human Nuclear transition protein 2 (TNP2) ELISA Kit

TNP2 were not found in human testicular RNA or human testis cDNA libraries. Finding homologous sequences in the human genome was greatly facilitated, however, by the finding that, in the boar and bull genomes, the genes for protamine-1 (PRM1) and Prm2 are clustered with Tnp2. Schluter et al. (1992) tested a genomic human cosmid clone containing the genes for both protamines and found that the TNP2 gene was within a distance of 7.5 kb 3-prime of the PRM2 gene. The gene was transcribed in human testicular RNA only at a level demonstrable by RT-PCR and RNase protection assay. In contrast to the TNP2 genes of mouse, rat, boar, and bull, the human TNP2 gene lacks the nucleotide sequence GCCATCAC in its 3-prime UTR.

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Human Nuclear transition protein 2 (TNP2) ELISA Kit has high sensitivity and excellent specificity for detection of Human TNP2. No significant cross-reactivity or interference between Human TNP2 and analogues was observed.

This Human Nuclear transition protein 2 (TNP2) ELISA Kit employs a two-site sandwich ELISA to quantitate TNP2 in samples. An antibody specific for TNP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.