His-tag Binding and Wash Buffer
<strong>His-tag Binding and Wash Buffer </strong>_x000D_ <strong>Catalog number:</strong> B2016383_x000D_ <strong>Lot number:</strong> Batch Dependent_x000D_ <strong>Expiration Date:</strong> Batch dependent_x000D_ <strong>Amount:</strong> 50 mL_x000D_ <strong>Molecular Weight or Concentration:</strong> N/A_x000D_ <strong>Supplied as:</strong> Solution_x000D_ <strong>Applications:</strong> a molecular tool for various biochemical applications_x000D_ <strong>Storage:</strong> RT_x000D_ <strong>Keywords:</strong> His Binding/Wash Buffer_x000D_ <strong>Grade:</strong> Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um._x000D_ _x000D_ <strong>References:</strong>_x000D_ 1: Hefti MH, Milder FJ, Boeren S, Vervoort J, van Berkel WJ. A His-tag based immobilization method for the preparation and reconstitution of apoflavoproteins Biochim Biophys Acta. 2003 Jan 20;1619(2):139-43._x000D_ 2: Al-Majdoub M, Opuni KF, Yefremova Y, Koy C, Lorenz P, El-Kased RF, Thiesen HJ, Glocker MO. A novel strategy for the rapid preparation and isolation of intact immune complexes from peptide mixtures J Mol Recognit. 2014 Sep;27(9):566-74._x000D_ 3: Zhang Y, Wu L, Yu J, Mei J, Yi Y, Chen J, Ying G. Preparation of recombinant human thymic stromal lymphopoietin protein Prep Biochem Biotechnol. 2017 Oct 21;47(9):934-938._x000D_ 4: de Marco A. Two-step metal affinity purification of double-tagged (NusA-His6) fusion proteins Nat Protoc. 2006;1(3):1538-43._x000D_ 5: Guo F, Zhu G. Presence and removal of a contaminating NADH oxidation activity in recombinant maltose-binding protein fusion proteins expressed in Escherichia coli Biotechniques. 2012 Apr;52(4):247-53._x000D_ 6: Keefe AD, Wilson DS, Seelig B, Szostak JW. One-step purification of recombinant proteins using a nanomolar-affinity streptavidin-binding peptide, the SBP-Tag Protein Expr Purif. 2001 Dec;23(3):440-6._x000D_ 7: Zhao JC, Zhao ZD, Wang W, Gao XM. Prokaryotic expression, refolding, and purification of fragment 450-650 of the spike protein of SARS-coronavirus Protein Expr Purif. 2005 Feb;39(2):169-74._x000D_ 8: Fernandez-Rodriguez J, Marlovits TC. Induced heterodimerization and purification of two target proteins by a synthetic coiled-coil tag Protein Sci. 2012 Apr;21(4):511-9._x000D_ 9: Sheikholvaezin A, Blomberg F, Ohrmalm C, Sjösten A, Blomberg J. Rational recombinant XMRV antigen preparation and bead coupling for multiplex serology in a suspension array Protein Expr Purif. 2011 Dec;80(2):176-84._x000D_ <a href="https://pubmed.ncbi.nlm.nih.gov/18189353">10: Liu F, Liao L, Chen J. Preparation of unglycosylated human caseinomacropeptide by engineering DAB Escherichia coli J Agric Food Chem. 2008 Feb 13;56(3):889-93. </a>_x000D_ _x000D_ <strong>Products Related to His-tag Binding and Wash Buffer can be found at</strong> <a href="https://moleculardepot.com/product-category/Chemicals/"> Chemicals</a>
Product Specifications
Short Description
Catalog Number: B2016383 (50 mL)
Weight
2.6
Length
3.3
Width
1.2
Height
1.2
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