Human Thyrotropin-releasing hormone (TRH) ELISA Kit

Thyrotropin releasing hormone, a hormone secreted by the hypothalamus that stimulates release of thyrotropin. TRH can also be detected in other areas of the body including the gastrointestinal system and pancreatic islets. Thyroid-stimulating hormone, also known as thyrotropin, is secreted from cells in the anterior pituitary called thyrotrophs. The most important controller of TSH secretion is thyroid-releasing hormone. TRH is produced by the hypothalamus in medial neurons of the paraventricular nucleus. Initially, it is synthesized as a 242 amino acid precursor polypeptide that contains 6 copies of the sequence -Gln-His-Pro-Gly-, flanked by di-basic peptides that are later processed through proteolysis to give the mature TRH molecule.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Thyrotropin-releasing hormone (TRH) ELISA Kit has high sensitivity and excellent specificity for detection of Human TRH. No significant cross-reactivity or interference between Human TRH and analogues was observed.

This Human Thyrotropin-releasing hormone (TRH) ELISA Kit employs a two-site sandwich ELISA to quantitate TRH in samples. An antibody specific for TRH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTRH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRH bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.