Human Transient receptor potential cation channel subfamily M member 1 (TRPM1) ELISA Kit

Transient receptor potential cation channel subfamily M member 1 is a protein similar to the transient receptor potential (Trp) calcium channel family members. The expression of this protein is inversely correlated with melanoma aggressiveness, suggesting that it suppresses melanoma metastasis. The expression of the TRPM1 gene is regulated by the Microphthalmia-associated transcription factor. Northern blot analysis of mouse tissues and cell lines revealed that melastatin was expressed as a 2.8-kb mRNA in normal eye and in 4 melanoma cell lines; its expression in each of the 4 cell lines was inversely proportional to metastatic potential. In 45 human melanocytic primary neoplasms examined by in situ hybridization, the loss of melastatin expression correlated with the thickness of the melanomas.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Transient receptor potential cation channel subfamily M member 1 (TRPM1) ELISA Kit has high sensitivity and excellent specificity for detection of Human TRPM1. No significant cross-reactivity or interference between Human TRPM1 and analogues was observed.

This Human Transient receptor potential cation channel subfamily M member 1 (TRPM1) ELISA Kit employs a two-site sandwich ELISA to quantitate TRPM1 in samples. An antibody specific for TRPM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTRPM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRPM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRPM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.