Human Tumor susceptibility gene 101 protein (TSG101) ELISA Kit

Tumor susceptibility gene 101, also known as TSG101, is a protein belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Tumor susceptibility gene 101 protein (TSG101) ELISA Kit has high sensitivity and excellent specificity for detection of Human TSG101. No significant cross-reactivity or interference between Human TSG101 and analogues was observed.

This Human Tumor susceptibility gene 101 protein (TSG101) ELISA Kit employs a two-site sandwich ELISA to quantitate TSG101 in samples. An antibody specific for TSG101 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTSG101 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TSG101 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TSG101 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.