Human UbiA prenyltransferase domain-containing protein 1 (UBIAD1) ELISA Kit

TERE1 expression in both superficial and muscle-invasive transitional cell carcinomas of bladder (TCCs) compared with normal human urothelium. Transfection of TERE1 into 2 human TCC cell lines inhibited cellular proliferation by 80 to 90%. In 1 line, TERE1 overexpression caused a change in morphology, with an apparent increase in intercellular contacts The deduced 338-amino acid protein has a calculated molecular mass of 36.8 kD. TERE1 has 5 putative transmembrane regions, an N-glycosylation site, and several putative phosphorylation sites. Northern blot and PCR analyses showed ubiquitous expression of 1.5- and 3-kb TERE1 transcripts. Immunohistochemical analysis of human urothelium revealed nuclear and cytoplasmic expression of TERE1.

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Human UbiA prenyltransferase domain-containing protein 1 (UBIAD1) ELISA Kit has high sensitivity and excellent specificity for detection of Human UBIAD1. No significant cross-reactivity or interference between Human UBIAD1 and analogues was observed.

This Human UbiA prenyltransferase domain-containing protein 1 (UBIAD1) ELISA Kit employs a two-site sandwich ELISA to quantitate UBIAD1 in samples. An antibody specific for UBIAD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUBIAD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UBIAD1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UBIAD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.