Human Trypsinogen activation peptide (TAP) ELISA Kit

Trypsinogen-activation peptide (TAP) is a small peptide that is released when trypsinogen is activated to trypsin. Under physiologic conditions, activation of trypsinogen occurs in the intestinal lumen and is catalyzed by enteropeptidase (formerly known as enterokinase) . In the intestinal lumen, TAP is quickly degraded by peptidases of the brush border membrane. In pancreatitis, trypsinogen is prematurely activated within pancreatic acinar cells, and TAP is released into the peripheral circulation. Although TAP is quickly excreted through the kidneys, with a circulating half-life of less than 8 min, significant increases in plasma and urine TAP concentrations have been reported in canine and human patients with acute pancreatitis. Trypsinogen activation peptide (TAP) may be an early marker of severe pancreatitis.

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Human Trypsinogen activation peptide (TAP) ELISA Kit has high sensitivity and excellent specificity for detection of Human USO1. No significant cross-reactivity or interference between Human USO1 and analogues was observed.

This Human Trypsinogen activation peptide (TAP) ELISA Kit employs a two-site sandwich ELISA to quantitate USO1 in samples. An antibody specific for USO1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUSO1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for USO1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of USO1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.