Human Histone demethylase UTY (UTY) ELISA Kit

This gene encodes a protein containing tetratricopeptide repeats which are thought to be involved in protein-protein interactions. This protein is a minor histocompatibility antigen which may induce graft rejection of male stem cell grafts. Alternative splicing results in multiple transcript variants encoding different isoforms.

UTY; DKFZp686L12190; UTY1; tetratricopeptide repeat protein; ubiquitous TPR motif protein UTY; ubiquitously transcribed TPR gene on Y chromosome; ubiquitously transcribed tetratricopeptide repeat gene; Y ch

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Histone demethylase UTY (UTY) ELISA Kit has high sensitivity and excellent specificity for detection of Human UTY. No significant cross-reactivity or interference between Human UTY and analogues was observed.

This Human Histone demethylase UTY (UTY) ELISA Kit employs a two-site sandwich ELISA to quantitate UTY in samples. An antibody specific for UTY has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUTY present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UTY is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UTY bound in the initial step. The color development is stopped and the intensity of the color is measured.

UTY; DKFZp686L12190; UTY1; tetratricopeptide repeat protein; ubiquitous TPR motif protein UTY; ubiquitously transcribed TPR gene on Y chromosome; ubiquitously transcribed tetratricopeptide repeat gene; Y ch

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.