Human UDP-glucuronic acid decarboxylase 1 (UXS1) ELISA Kit

UDP-glucuronate decarboxylase (UGD; EC 4.1.1.35) catalyzes the formation of UDP-xylose from UDP-glucuronate. UDP-xylose is then used to initiate glycosaminoglycan biosynthesis on the core protein of proteoglycans. The topology of the deduced 420-amino acid protein was consistent with a type II transmembrane protein. Northern blot analysis of rat tissues detected a single UGD transcript with highest levels in heart, brain, and testes. Substantial levels were also detected in kidney, liver and lung, and lower levels in spleen and skeletal muscle. Western blot analysis demonstrated highest levels of the protein in kidney, liver, and brain, with negligible staining in heart, spleen, skeletal muscle, lung, and testes. Subcellular studies and histochemistry localized the protein to the perinuclear Golgi.

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Human UDP-glucuronic acid decarboxylase 1 (UXS1) ELISA Kit has high sensitivity and excellent specificity for detection of Human UXS1. No significant cross-reactivity or interference between Human UXS1 and analogues was observed.

This Human UDP-glucuronic acid decarboxylase 1 (UXS1) ELISA Kit employs a two-site sandwich ELISA to quantitate UXS1 in samples. An antibody specific for UXS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyUXS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UXS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UXS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.