Human Vasohibin-1 (VASH1) ELISA Kit

Vasohibin protein contains a cluster of basic amino acids in its C terminus and no classic secretion signal sequence. Vasohibin expression was robust between embryonic weeks 6 to 12 in heart, brain, kidney, lung, skeletal muscle, and whole embryo. Immunohistochemical analysis detected vasohibin at the endothelial layer of placenta and brain. The deduced protein contains 365 amino acids. RT-PCR ELISA detected intermediate expression in adult heart, brain, lung, liver, kidney, ovary, and spinal cord, as well as in fetal brain. Lower expression was detected in skeletal muscle, testis, pancreas, and fetal liver, and little to no expression was detected in spleen. All specific adult brain regions examined showed low to moderate expression.

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Human Vasohibin-1 (VASH1) ELISA Kit has high sensitivity and excellent specificity for detection of Human VASH1. No significant cross-reactivity or interference between Human VASH1 and analogues was observed.

This Human Vasohibin-1 (VASH1) ELISA Kit employs a two-site sandwich ELISA to quantitate VASH1 in samples. An antibody specific for VASH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVASH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VASH1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VASH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.