Human VGF nerve growth factor inducible (VGF) ELISA Kit

VGF or VGF nerve growth factor inducible is a protein and neuropeptide that may play a role in regulating energy homeostasis, metabolism and synaptic plasticity. The protein was first discovered in 1985 by Lewi et al. in an experiment with PC12 cells and its name is non-acronymic. VGF gene encodes a precursor which is divided by proteolysis to polypeptides of different mass, which have a variety of functions, the best studied of which is the role of TLQP-21 in the control of appetite and inflammation. The expression of VGF and VGF-derived peptides is detected in a subset of neurons in the central and peripheral nervous systems and specific populations of endocrine cells in the adenohypophysis, adrenal medulla, gastrointestinal tract, and pancreas.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human VGF nerve growth factor inducible (VGF) ELISA Kit has high sensitivity and excellent specificity for detection of Human VGF. No significant cross-reactivity or interference between Human VGF and analogues was observed.

This Human VGF nerve growth factor inducible (VGF) ELISA Kit employs a two-site sandwich ELISA to quantitate VGF in samples. An antibody specific for VGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VGF is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VGF bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.