Human Vasoactive intestinal polypeptide receptor 1 (VIPR1) ELISA Kit

Vasoactive intestinal peptide receptor 1 is expressed in the brain (cerebral cortex, hippocampus, amygdala), lung, prostate, peripheral blood leukocytes, liver, small intestine, heart, spleen, placenta, kidney, thymus and testis . This gene encodes a receptor for vasoactive intestinal peptide, a small neuropeptide. Vasoactive intestinal peptide is involved in smooth muscle relaxation, exocrine and endocrine secretion, and water and ion flux in lung and intestinal epithelia. Its actions are effected through integral membrane receptors associated with a guanine nucleotide binding protein which activates adenylate

VIPR1; FLJ41949; HVR1; ; PACAP-R-2; RDC1; V1RG; VAPC1; VIPR; VIRG; VPAC1; VPCAP1R; PACAP type receptor; VIP receptor; type I; pituitary adenylate cyclase activating polypeptide receptor; type

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Vasoactive intestinal polypeptide receptor 1 (VIPR1) ELISA Kit has high sensitivity and excellent specificity for detection of Human VIPR1. No significant cross-reactivity or interference between Human VIPR1 and analogues was observed.

This Human Vasoactive intestinal polypeptide receptor 1 (VIPR1) ELISA Kit employs a two-site sandwich ELISA to quantitate VIPR1 in samples. An antibody specific for VIPR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVIPR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VIPR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VIPR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

VIPR1; FLJ41949; HVR1; ; PACAP-R-2; RDC1; V1RG; VAPC1; VIPR; VIRG; VPAC1; VPCAP1R; PACAP type receptor; VIP receptor; type I; pituitary adenylate cyclase activating polypeptide receptor; type

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.