Human Vitamin K epoxide reductase complex subunit 1 (VKORC1) ELISA Kit

Vitamin K is essential for blood clotting but must be enzymatically activated. This enzymatically activated form of vitamin K is a reduced form required for the carboxylation of glutamic acid residues in some blood-clotting proteins. VKORC1 is responsible for reducing vitamin K 2,3-epoxide to the enzymatically activated form. Fatal bleeding can be caused by vitamin K deficiency and by the vitamin K antagonist warfarin, and it is the product of this gene that is sensitive to warfarin. In humans, mutations in this gene can be associated with deficiencies in vitamin-K-dependent clotting factors and, in humans and rats, with warfarin resistance. Two pseudogenes have been identified on chromosome 1 and the X chromosome. Two alternatively spliced transcripts encoding different isoforms have been described.

VKORC1; EDTP308; FLJ00289; IMAGE3455200; MGC2694; MST134; MST576; VKCFD2; VKOR; phylloquinone epoxide reductase; vitamin K dependent clotting factors deficiency 2; vitamin K1 epoxide reductase (warfarin-sensi

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Vitamin K epoxide reductase complex subunit 1 (VKORC1) ELISA Kit has high sensitivity and excellent specificity for detection of Human VKORC1. No significant cross-reactivity or interference between Human VKORC1 and analogues was observed.

This Human Vitamin K epoxide reductase complex subunit 1 (VKORC1) ELISA Kit employs a two-site sandwich ELISA to quantitate VKORC1 in samples. An antibody specific for VKORC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVKORC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VKORC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VKORC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

VKORC1; EDTP308; FLJ00289; IMAGE3455200; MGC2694; MST134; MST576; VKCFD2; VKOR; phylloquinone epoxide reductase; vitamin K dependent clotting factors deficiency 2; vitamin K1 epoxide reductase (warfarin-sensi

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.