Human Vacuolar protein sorting-associated protein 37A (VPS37A) ELISA Kit

HCRP1 encodes a deduced 397-amino acid protein. Northern blot analysis showed ubiquitous expression of an approximately 2-kb HCRP1 transcript, with most abundant expression in liver. Western blot analysis showed reduced HCRP1 expression in 6 of 8 HCC tissues, but higher expression levels of HCRP1 in adjacent normal tissues in all 8 cases. Transient transfection of GFP-HCRP1 fusion constructs showed that HCRP1 is localized throughout the cell, but predominantly in the nucleus.Pull-down assays demonstrated that the interaction between HCRP1 and TSG101 occurs through the mod (r) domain. By size exclusion chromatography, HCRP1 cofractionated with TSG101 and VPS28. Depletion of TSG101 by siRNA treatment resulted in reduction in HCRP1 levels in HeLa cells.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Human Vacuolar protein sorting-associated protein 37A (VPS37A) ELISA Kit has high sensitivity and excellent specificity for detection of Human VPS37A. No significant cross-reactivity or interference between Human VPS37A and analogues was observed.

This Human Vacuolar protein sorting-associated protein 37A (VPS37A) ELISA Kit employs a two-site sandwich ELISA to quantitate VPS37A in samples. An antibody specific for VPS37A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVPS37A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VPS37A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VPS37A bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.