Human Serine/threonine-protein kinase VRK1 (VRK1) ELISA Kit

Serine/threonine-protein kinase VRK2 is an a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. This gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, leukocytes, fetal liver, and carcinomas. Its protein localizes to the endoplasmic reticulum and has been shown to phosphorylate casein and undergo autophosphorylation. While several transcript variants may exist for this gene, the full-length nature of only one has been biologically validated to date.Widely expressed. Highly expressed in fetal liver, skeletal muscle, pancreas, heart, peripheral blood leukocytes and testis.

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Human Serine/threonine-protein kinase VRK1 (VRK1) ELISA Kit has high sensitivity and excellent specificity for detection of Human VRK1. No significant cross-reactivity or interference between Human VRK1 and analogues was observed.

This Human Serine/threonine-protein kinase VRK1 (VRK1) ELISA Kit employs a two-site sandwich ELISA to quantitate VRK1 in samples. An antibody specific for VRK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVRK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VRK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VRK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.