Human V-set and immunoglobulin domain-containing protein 2 (VSIG2) ELISA Kit

VSIG2 gene contains 7 conserved exons, with each Ig domain encoded by 2 half-domain exons with unusual splicing junctions.The deduced 325-amino acid CTH protein, which is 29% similar to GPA33, contains a signal peptide, an extracellular V-type Ig-like domain followed by a C2-type Ig-like domain, a transmembrane region devoid of charged residues, and a cytoplasmic tail. There are multiple N-glycosylation sites in the extracellular region and phosphorylation sites in the cytoplasmic tail. Northern blot analysis revealed CTH expression in stomach, colon, prostate, trachea, and thyroid gland, with lower levels in bladder and lung. CTH expression was not detected in thymus, although CTH could be amplified by PCR from a thymus cDNA library. The Ctx-like molecules are novel members of the Ig superfamily

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Human V-set and immunoglobulin domain-containing protein 2 (VSIG2) ELISA Kit has high sensitivity and excellent specificity for detection of Human VSIG2. No significant cross-reactivity or interference between Human VSIG2 and analogues was observed.

This Human V-set and immunoglobulin domain-containing protein 2 (VSIG2) ELISA Kit employs a two-site sandwich ELISA to quantitate VSIG2 in samples. An antibody specific for VSIG2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyVSIG2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VSIG2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VSIG2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.