Human WAP four-disulfide core domain protein 5 (WFDC5) ELISA Kit

WFDC5 encodes a member of the WAP-type four-disulfide core (WFDC) domain family. Most WFDC proteins contain only one WFDC domain, and this encoded protein contains two WFDC domains. The WFDC domain, or WAP signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. The deduced 123-amino acid protein contains an N-terminal signal peptide, followed by 2 WAP domains. Each WAP domain contains 8 cysteines that form conserved disulfide bridges, and each WAP domain forms a proposed protease-binding loop and a beta sheet. The mature 99-amino acid WAP1 protein has a calculated molecular mass of 10.9 kD.

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Human WAP four-disulfide core domain protein 5 (WFDC5) ELISA Kit has high sensitivity and excellent specificity for detection of Human WFDC5. No significant cross-reactivity or interference between Human WFDC5 and analogues was observed.

This Human WAP four-disulfide core domain protein 5 (WFDC5) ELISA Kit employs a two-site sandwich ELISA to quantitate WFDC5 in samples. An antibody specific for WFDC5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWFDC5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WFDC5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WFDC5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.