Human Wnt inhibitory factor 1 (WIF1) ELISA Kit

WNT proteins are extracellular signaling molecules involved in the control of embryonic development. This gene encodes a secreted protein, which binds WNT proteins and inhibits their activities. This protein contains a WNT inhibitory factor (WIF) domain and 5 epidermal growth factor (EGF) -like domains. It may be involved in mesoderm segmentation. This protein is found to be present in fish, amphibia and mammals.The deduced 379-amino acid WIF1 secreted protein contains an N-terminal signal sequence, a 150-amino acid WIF domain, 5 epidermal growth factor (EGF) -like repeats that are similar to those of tenascin, and a C-terminal hydrophilic domain of approximately 45 amino acids. Overexpression of human WIF1 in frog embryos affected somitogenesis depending on the site and timing of WIF1 injection. .

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Human Wnt inhibitory factor 1 (WIF1) ELISA Kit has high sensitivity and excellent specificity for detection of Human WIF1. No significant cross-reactivity or interference between Human WIF1 and analogues was observed.

This Human Wnt inhibitory factor 1 (WIF1) ELISA Kit employs a two-site sandwich ELISA to quantitate WIF1 in samples. An antibody specific for WIF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWIF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WIF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WIF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.