Human WNT1-inducible-signaling pathway protein 2 (WISP2) ELISA Kit

WISP2 encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The encoded protein lacks the CT domain which is implicated in dimerization and heparin binding. It is 72% identical to the mouse protein at the amino acid level. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. Its expression in colon tumors is reduced while the other two WISP members are overexpressed in colon tumors. It is expressed at high levels in bone tissue, and may play an important role in modulating bone turnover.

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Human WNT1-inducible-signaling pathway protein 2 (WISP2) ELISA Kit has high sensitivity and excellent specificity for detection of Human WISP2. No significant cross-reactivity or interference between Human WISP2 and analogues was observed.

This Human WNT1-inducible-signaling pathway protein 2 (WISP2) ELISA Kit employs a two-site sandwich ELISA to quantitate WISP2 in samples. An antibody specific for WISP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWISP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WISP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WISP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.