Myc tag, AlpHcAbs® Mouse IgG1
Anti-Myc tag, AlpHcAbs® Mouse IgG1 antibody is designed for detecting Myc tag fusion protein specifically. Anti-Myc tag, AlpHcAbs® Mouse IgG1 antibody is recombinant fab of alpaca IgG1 fused to mouse IgG1 Fc. Based on western blot and ELISA, Anti-Myc tag, AlpHcAbs® Mouse IgG1 antibody reacts with the Myc tag sequence (EQKLISEEDL) selectively, no reactivity with other proteins.
Product Specifications
Host
Alpaca
Species Reactivity
Universal
Immunogen
Myc tag fusion protein
Conjugation
Non-conjugated
Type
Alpaca monoclonal Antibody
Applications
WB, ICC/IF, ELISA, IP, FC
Concentration
1 mg/mL
Dilution
WB: 1:10,000-1:50,000
;ICC/IF: 1:200-1:1000
;ELISA: 1:10,000-1:50,000
;IP: 1-2ug/sample
;FC: 1µg for 106 cells
;Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
Purity
Recombinant Expression and Affinity purified
Form
Liquid
Buffer
Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol
Storage Conditions
Store at –20°C, (Avoid freeze / thaw cycles), Stable for 12 months at -20°C
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