Rat Tumor necrosis factor soluble receptor II (TNFsR-II) ELISA Kit

The protein encoded by TNFsR-II is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. The function of IAPs in TNF-receptor signalling is unknown, however, c-IAP1 is thought to potentiate TNF-induced apoptosis by the ubiquitination and degradation of TNF-receptor-associated factor 2, which mediates anti-apoptotic signals. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways. [provided by RefSeq, Jul 2008]

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Rat Tumor necrosis factor soluble receptor II (TNFsR-II) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TNFsR-II. No significant cross-reactivity or interference between Rat TNFsR-II and analogues was observed.

This Rat Tumor necrosis factor soluble receptor II (TNFsR-II) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFsR-II in samples. An antibody specific for TNFsR-II has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFsR-II present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFsR-II is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFsR-II bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.