Rat Cardiac isoform of Tropnin T (cTnT) ELISA Kit

TNNT2 is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well

TNNT2; CMH2; CMPD2; MGC3889; RCM3; TnTC; cTnT; OTTHUMP00000033864; OTTHUMP00000033867; cardiomyopathy; dilated 1D (autosomal dominant) ; cardiomyopathy; hypertrophic 2; troponin T type 2; cardiac; troponin T; ca

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Rat Cardiac isoform of Tropnin T (cTnT) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TNNT2. No significant cross-reactivity or interference between Rat TNNT2 and analogues was observed.

This Rat Cardiac isoform of Tropnin T (cTnT) ELISA Kit employs a two-site sandwich ELISA to quantitate TNNT2 in samples. An antibody specific for TNNT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNNT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNNT2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNNT2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

TNNT2; CMH2; CMPD2; MGC3889; RCM3; TnTC; cTnT; OTTHUMP00000033864; OTTHUMP00000033867; cardiomyopathy; dilated 1D (autosomal dominant) ; cardiomyopathy; hypertrophic 2; troponin T type 2; cardiac; troponin T; ca

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.