Rat Protein Tob1 (TOB1) ELISA Kit

TOB2 belongs to the TOB/BTG1 family of antiproliferative proteins, which are involved in the regulation of cell cycle progression.The deduced 344-amino acid TOB2 polypeptide has a calculated molecular mass of 37 kD. It shares 61% overall sequence homology with the TOB1 protein, and both proteins contain a putative nuclear localization signal in the conserved N-terminal domain. Immunofluorescence analysis revealed localization almost exclusively in the cytoplasm. Northern blot analysis of human adult tissues detected ubiquitous expression of a 4.1-kb transcript, with relatively high expression in skeletal muscle, thymus, and ovary. In situ hybridization in several mouse tissues demonstrated intense hybridization in ovary, with characteristic expression in oocytes. Immunoblot analysis revealed a 43-kD protein.

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Rat Protein Tob1 (TOB1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TOB1. No significant cross-reactivity or interference between Rat TOB1 and analogues was observed.

This Rat Protein Tob1 (TOB1) ELISA Kit employs a two-site sandwich ELISA to quantitate TOB1 in samples. An antibody specific for TOB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTOB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TOB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TOB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.