What is the catalog number for Rat Tryptophan Hydroxylase (TPH) ELISA Kit?

The catalog number for Rat Tryptophan Hydroxylase (TPH) ELISA Kit is KTE100042.

What sizes are available for Rat Tryptophan Hydroxylase (TPH) ELISA Kit?

Rat Tryptophan Hydroxylase (TPH) ELISA Kit is available in the following size(s): 48 Tests, 96 Tests, 5x 96 Tests, 50x 96 Tests.

How many pack sizes does Rat Tryptophan Hydroxylase (TPH) ELISA Kit come in?

Rat Tryptophan Hydroxylase (TPH) ELISA Kit is available in 4 different pack sizes.

Is Rat Tryptophan Hydroxylase (TPH) ELISA Kit in stock?

Rat Tryptophan Hydroxylase (TPH) ELISA Kit is currently made to order / not held in immediate stock. Contact us for lead time.

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.

What is the recommended incubation time for this antibody?

A suggested incubation time is provided in the recommended protocol on the datasheet, but may need adjusting depending on your sample type and detection system.

What should I check when this product first arrives?

Inspect the package immediately for transportation damage, ice loss, leakage, or breakage. Report any issues right away with photos and your order reference; our specialists will determine whether the product is still usable or needs replacing.

Rat Tryptophan Hydroxylase (TPH) ELISA Kit

This Rat Tryptophan Hydroxylase (TPH) ELISA Kit employs a two-site sandwich ELISA to quantitate TPH1.

Product Specifications

Background

TPH2 encodes a member of the pterin-dependent aromatic acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. The human genome contains two related tryptophan hydroxylases, one on chromosome 11p15-p14 and one on chromosome 12q21. This gene is expressed predominantly in the brain stem. Mutations in this gene may be associated with psychiatric diseases such as bipolar affective disorder and major depression. This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by the mouse ortholog.

CAS Number

7732-18-5

Product Name Alternative

TPH1; MGC119994; TPRH; TRPH; L-tryptophan hydroxylase; indoleacetic acid-5-hydroxylase; tryptophan hydroxylase (tryptophan 5-monooxygenase)

Gene ID

100009100

Accession Number

P17290

Reactivity

Rat

Tag

TPH1

Type

Cell analysis

Applications

ELISA

Detection Method

Colorimetric

Assay Type

Sandwich ELISA (quantitative)

Assay Performance Time

Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Sample Type

Cell culture supernatants#Serum#Plasma#Other biological fluids

Limit Of Detection

Please inquire

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Features & Benefits

Rat Tryptophan Hydroxylase (TPH) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TPH1. No significant cross-reactivity or interference between Rat TPH1 and analogues was observed.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Applications Notes

This Rat Tryptophan Hydroxylase (TPH) ELISA Kit employs a two-site sandwich ELISA to quantitate TPH1 in samples. An antibody specific for TPH1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTPH1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPH1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPH1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Other Product Names

TPH1; MGC119994; TPRH; TRPH; L-tryptophan hydroxylase; indoleacetic acid-5-hydroxylase; tryptophan hydroxylase (tryptophan 5-monooxygenase)

Tested Applications

ELISA Testing

Recommended Usage

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.