Rat Thyroid-Peroxidase (TPO) ELISA Kit

This Rat Thyroid-Peroxidase (TPO) ELISA Kit employs a two-site sandwich ELISA to quantitate TPO.

Product Specifications

Background

Membrane-bound glycoprotein; iodinates tyrosine residues in thyroglobulin to generate biologically active thyroid hormones T3 and T4 [RGD, Feb 2006]

CAS Number

7732-18-5

Product Name Alternative

TPO; MSA; TDH2A; TPX; OTTHUMP00000115536; thyroid microsomal antigen; thyroperoxidase

Accession Number

P14650

Reactivity

Rat

Tag

TPO

Type

Cell analysis

Applications

ELISA

Detection Method

Colorimetric

Assay Type

Sandwich ELISA (quantitative)

Assay Performance Time

Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Sample Type

Cell culture supernatants#Serum#Plasma#Other biological fluids

Limit Of Detection

Please inquire

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Features & Benefits

Rat Thyroid-Peroxidase (TPO) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TPO. No significant cross-reactivity or interference between Rat TPO and analogues was observed.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Applications Notes

This Rat Thyroid-Peroxidase (TPO) ELISA Kit employs a two-site sandwich ELISA to quantitate TPO in samples. An antibody specific for TPO has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTPO present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPO is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPO bound in the initial step. The color development is stopped and the intensity of the color is measured.

Other Product Names

TPO; MSA; TDH2A; TPX; OTTHUMP00000115536; thyroid microsomal antigen; thyroperoxidase

Tested Applications

ELISA Testing

Recommended Usage

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.