Rat Transient receptor potential cation channel subfamily M member 4 (TRPM4) ELISA Kit

TRPM5 expression in several human and rodent cell lines, including murine neuronal cells, Burkitt lymphoma cells, murine B-lymphoma cells, HeLa cells, murine pancreatic beta cells, rat beta cells, and human pancreatic islets. No endogenous TRPM5 was detected in human embryonic kidney (HEK) -293 cells. Among the differentially expressed clones, one encoded Trpm5. In situ hybridization detected Trpm5 expression restricted to mouse taste tissue. In taste cells, Trpm5 was coexpressed with taste-signaling molecules such as alpha-gustducin, G protein gamma-13 subunit, phospholipase C-beta-2 (PLCB2), and inositol 1,4,5-trisphosphate receptor type III.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Rat Transient receptor potential cation channel subfamily M member 4 (TRPM4) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TRPM4. No significant cross-reactivity or interference between Rat TRPM4 and analogues was observed.

This Rat Transient receptor potential cation channel subfamily M member 4 (TRPM4) ELISA Kit employs a two-site sandwich ELISA to quantitate TRPM4 in samples. An antibody specific for TRPM4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTRPM4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRPM4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRPM4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.