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ZO3 Polyclonal Antibody

Rabbit Anti-ZO3 Polyclonal Antibody

Product Specifications

Background

The protein encoded by TJP3 is a member of the membrane-associated guanylate kinase-like (MAGUK) protein family which is characterized by members having multiple PDZ domains, a single SH3 domain, and a single guanylate kinase-like (GUK) -domain. In addition, members of the zonula occludens protein subfamily have an acidic domain, a basic arginine-rich region, and a proline-rich domain. The protein encoded by TJP3 plays a role in the linkage between the actin cytoskeleton and tight-junctions and also sequesters cyclin D1 at tight junctions during mitosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms. TJP3 has a partial pseudogene on chromosome 1.

CAS Number

9007-83-4

Product Name Alternative

Tight junction protein ZO-3; Tight junction protein 3; Zona occludens protein 3; Zonula occludens protein 3

Gene ID

27134

Accession Number

O95049

Reactivity

Human, Mouse, Monkey

Immunogen

Synthesized peptide derived from part region of human ZO3 protein

Clonality

Polyclonal

Tag

ZO3

Applications

WB, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

102kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

The antibody detects endogenous levels of ZO3 protein

Product Datasheet

https://www.abbkine.com/datasheet/ABP60993.pdf

Observed Molecular Weight

102

Formulation

Liquid solution

Buffer pH

PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000; ELISA 1:5000-20000

Other Product Names

Tight junction protein ZO-3; Tight junction protein 3; Zona occludens protein 3; Zonula occludens protein 3

Tested Applications

WB, IHC, IF, IP, ELISA

Host or Source

Rabbit

Accession Number URL

http://www.uniprot.org/uniprot/O95049

Isotype

Rabbit IgG

Available Sizes

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