SPAG8 Polyclonal Antibody

The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. Sperm Associated Antigen 8 encoded by SPAG8 is recognized by sperm agglutinating antibodies from an infertile woman. Sperm Associated Antigen 8 is localized in germ cells of the testis at all stages of spermatogenesis and is localized to the acrosomal region of mature spermatozoa. Sperm Associated Antigen 8 interacts with ACT (activator of CREM in testis) and may play a role in CREM (cAMP response element modulator) -ACT-mediated gene transcription during spermatogenesis. Sperm Associated Antigen 8 may also play a role in spermatogenesis by regulating microtubule formation and cell division. Alternatively spliced variants that encode different protein isoforms have been described but the full-length sequences of only two have been determined.

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Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000; ELISA 1:5000-20000