RT28 Polyclonal Antibody
Rabbit Anti-RT28 Polyclonal Antibody
Product Specifications
Background
Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. MRPS28 encodes a 28S subunit protein that has been called mitochondrial ribosomal protein S35 in the literature.
CAS Number
9007-83-4
Product Name Alternative
28S ribosomal protein S28, mitochondrial; MRP-S28; S28mt; 28S ribosomal protein S35, mitochondrial; MRP-S35; S35mt; Mitochondrial small ribosomal subunit protein bS1m
Gene ID
28957
Accession Number
Q9Y2Q9
Reactivity
Human, Mouse
Immunogen
Synthesized peptide derived from part region of human RT28 protein at amino acid sequence of 110-190
Clonality
Polyclonal
Tag
RT28
Applications
WB, ELISA
Purification Method
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Molecular Weight
20kD
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.
Notes
The antibody detects endogenous levels of RT28 protein
Product Datasheet
https://www.abbkine.com/datasheet/ABP60279.pdf
Observed Molecular Weight
20
Formulation
Liquid solution
Buffer pH
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Applications Notes
Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000; ELISA 1:5000-20000
Other Product Names
28S ribosomal protein S28, mitochondrial; MRP-S28; S28mt; 28S ribosomal protein S35, mitochondrial; MRP-S35; S35mt; Mitochondrial small ribosomal subunit protein bS1m
Tested Applications
WB, IHC, IF, IP, ELISA
Host or Source
Rabbit
Accession Number URL
http://www.uniprot.org/uniprot/Q9Y2Q9
Isotype
Rabbit IgG
Available Sizes
Curated Selection
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