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RT28 Polyclonal Antibody

Rabbit Anti-RT28 Polyclonal Antibody

Product Specifications

Background

Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. MRPS28 encodes a 28S subunit protein that has been called mitochondrial ribosomal protein S35 in the literature.

CAS Number

9007-83-4

Product Name Alternative

28S ribosomal protein S28, mitochondrial; MRP-S28; S28mt; 28S ribosomal protein S35, mitochondrial; MRP-S35; S35mt; Mitochondrial small ribosomal subunit protein bS1m

Gene ID

28957

Accession Number

Q9Y2Q9

Reactivity

Human, Mouse

Immunogen

Synthesized peptide derived from part region of human RT28 protein at amino acid sequence of 110-190

Clonality

Polyclonal

Tag

RT28

Applications

WB, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

20kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

The antibody detects endogenous levels of RT28 protein

Product Datasheet

https://www.abbkine.com/datasheet/ABP60279.pdf

Observed Molecular Weight

20

Formulation

Liquid solution

Buffer pH

PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000; ELISA 1:5000-20000

Other Product Names

28S ribosomal protein S28, mitochondrial; MRP-S28; S28mt; 28S ribosomal protein S35, mitochondrial; MRP-S35; S35mt; Mitochondrial small ribosomal subunit protein bS1m

Tested Applications

WB, IHC, IF, IP, ELISA

Host or Source

Rabbit

Accession Number URL

http://www.uniprot.org/uniprot/Q9Y2Q9

Isotype

Rabbit IgG

Available Sizes

Curated Selection

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